Rojana Sukchawalit, Ph.D.

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Email: rojana@cri.or.th

Office: Biomedical Science Building, 8th floor, Zone A

Phone: (66)2 553 8555 ext. 8364

Research Interests:

Due to both the necessity of iron for physiological functions and also the toxicity of excess iron resulting from the formation of iron-catalyzed reactive oxygen species, therefore, iron homeostasis is tightly regulated in bacteria to ensure survival. The research aims at characterization of iron-sensing transcriptional regulators, Fur (ferric uptake regulator), RirA (rhizobial iron regulator) and Irr (iron response regulator) from a phyto pathogen Agrobacterium tumefaciens, in order to better understand mechanisms used by bacteria to satisfy their iron demand and prevent iron-induced toxicity.

It is also of interest to search for novel physiological roles of RirA and Irr beyond the iron regulation and study interplay between RirA, Irr and other transcriptional regulators to mediate the regulation of gene expression in response to diverse stressful conditions. The research will provide insight into functional roles as well as the impact of these regulators on the survival of A. tumefaciens during plant-pathogen interactions. RirA and Irr are found exclusively in members of alpha-proteobacteria including plant, animal and human pathogens. Therefore, the information on the distinct gene regulation mediated by RirA and Irr may provide a potential target for controlling diseases.

Selected Publications:

  1. Regulation of the Cobalt/Nickel Efflux Operon dmeRF in Agrobacterium tumefaciens and a Link between the Iron-Sensing Regulator RirA and Cobalt/Nickel Resistance.
    Dokpikul T, Chaoprasid P, Saninjuk K, Sirirakphaisarn S, Johnrod J, Nookabkaew S, Sukchawalit R, Mongkolsuk S
    Appl Environ Microbiol82p4732-42(2016 Aug 1)
  2. Agrobacterium tumefaciens Zur Regulates the High-Affinity Zinc Uptake System TroCBA and the Putative Metal Chaperone YciC, along with ZinT and ZnuABC, for Survival under Zinc-Limiting Conditions.
    Chaoprasid P, Dokpikul T, Johnrod J, Sirirakphaisarn S, Nookabkaew S, Sukchawalit R, Mongkolsuk S
    Appl Environ Microbiol82p3503-14(2016 Jun 15)
  3. Control of zinc homeostasis in Agrobacterium tumefaciens via zur and the zinc uptake genes znuABC and zinT.
    Bhubhanil S, Sittipo P, Chaoprasid P, Nookabkaew S, Sukchawalit R, Mongkolsuk S
    Microbiology160p2452-63(2014 Nov)
  4. Roles of Agrobacterium tumefaciens membrane-bound ferritin (MbfA) in iron transport and resistance to iron under acidic conditions.
    Bhubhanil S, Chamsing J, Sittipo P, Chaoprasid P, Sukchawalit R, Mongkolsuk S
    Microbiology160p863-71(2014 May)
  5. Identification of amino acid residues important for the function of Agrobacterium tumefaciens Irr protein.
    Bhubhanil S, Ruangkiattikul N, Niamyim P, Chamsing J, Ngok-Ngam P, Sukchawalit R, Mongkolsuk S
    FEMS Microbiol Lett335p68-77(2012 Oct)
  6. Agrobacterium tumefaciens membrane-bound ferritin plays a role in protection against hydrogen peroxide toxicity and is negatively regulated by the iron response regulator.
    Ruangkiattikul N, Bhubhanil S, Chamsing J, Niamyim P, Sukchawalit R, Mongkolsuk S
    FEMS Microbiol Lett329p87-92(2012 Apr)
  7. Roles of Agrobacterium tumefaciens RirA in iron regulation, oxidative stress response, and virulence.
    Ngok-Ngam P, Ruangkiattikul N, Mahavihakanont A, Virgem SS, Sukchawalit R, Mongkolsuk S
    J Bacteriol191p2083-90(2009 Apr)
  8. Agrobacterium tumefaciens fur has important physiological roles in iron and manganese homeostasis, the oxidative stress response, and full virulence.
    Kitphati W, Ngok-Ngam P, Suwanmaneerat S, Sukchawalit R, Mongkolsuk S
    Appl Environ Microbiol73p4760-8(2007 Aug)
  9. Protection of Xanthomonas against arsenic toxicity involves the peroxide-sensing transcription regulator OxyR.
    Sukchawalit R, Prapagdee B, Charoenlap N, Vattanaviboon P, Mongkolsuk S
    Res Microbiol156p30-4(2005 Jan-Feb)

Full list of publications